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Water Potential · Paper 2 — Free Biology HL Practice Questions

1MasterySAQ-SOsmosis and water movement in cells6 marksPaper 2~9 min
A student investigates osmosis using cylinders of potato (Solanum tuberosum) tissue. She cuts five cylinders of equal size and mass, then places each in a different concentration of sucrose solution. After 30 minutes, she removes the cylinders, blots them dry, and measures their mass again. Her results are shown below. Sucrose concentration (mol dm−3)(\text{mol dm}^{-3}) — 0.00.0 — 0.20.2 — 0.40.4 — 0.60.6 — 0.80.8 Percentage change in mass (%)(\%) — +12+12 — +5+5 — −2-2 — −8-8 — −15-15
(a)
Describe the relationship between sucrose concentration and the percentage change in mass of the potato cylinders. [2 marks]
(b)
Using the data, estimate the sucrose concentration at which the potato tissue would show no net change in mass. [1 mark]
(c)
State what the concentration estimated in (b) represents in terms of the water potential of the potato cells. [1 mark]
(d)
The student repeats the experiment but does not blot the cylinders dry before re-weighing. Evaluate how this procedural change would affect the reliability of her conclusion about the isotonic concentration. [2 marks]
diagram

Solutions

2MasterySAQ-SOsmosis and water movement in cells5 marksPaper 2~8 min
A researcher studies water uptake in the freshwater plant Elodea canadensis. She places a leaf in a 10%10\% sucrose solution and observes the cells under a microscope. Initially, the chloroplasts are distributed throughout each cell. After 10 minutes, the chloroplasts are clumped in the centre of each cell, and the cell membrane has pulled away from the cell wall.
(a)
State the process by which water leaves the cell, causing the cell membrane to pull away from the cell wall. [1 mark]
(b)
The initial volume of the cell sap (vacuole) is 2000 μm32000\,\mu\text{m}^3 and the final volume after the membrane pulls away from the cell wall is 1400 μm31400\,\mu\text{m}^3. Calculate the percentage decrease in the volume of the cell sap. [2 marks]
(c)
The researcher replaces the 10%10\% sucrose solution with distilled water and waits 10 minutes. Explain whether the cells will return to their original turgid state, with reference to water potential and pressure potential. [2 marks]
diagram

Solutions

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